基因数据处理53之cs-bwamem集群版运行paird-end(1千万条100bp的reads)

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  1. art:
    art_illumina -ss HS20 -i GRCH38BWAindex/GRCH38chr1L3556522.fna -p -l 100 -m 200 -s 10  -c 10000000 -o g38L100c10000000Nhs20Paired

2.fastq上传到hdfs

    spark-submit  --class cs.ucla.edu.bwaspark.BWAMEMSpark --master spark://219.219.220.149:7077  /home/hadoop/xubo/tools/cloud-scale-bwamem-0.2.1/target/cloud-scale-bwamem-0.2.0-assembly.jar upload-fastq   1 21 g38L100c10000000Nhs20Paired1.fq g38L100c10000000Nhs20Paired2.fq /xubo/data/alignment/cs-bwamem/fastq/g38L100c10000000Nhs20Paired12.fastq

3.配置:
需要将fasta dispatch到各个节点,每个节点需要有cs-bwamem的jar包,jni搞全局路径

4.align:

    spark-submit --executor-memory 4g --class cs.ucla.edu.bwaspark.BWAMEMSpark --total-executor-cores 20 --master spark://219.219.220.149:7077  --conf spark.driver.host=219.219.220.149 --conf spark.driver.cores=4 --conf spark.driver.maxResultSize=4g --conf spark.storage.memoryFraction=0.7  --conf spark.akka.threads=2 --conf spark.akka.frameSize=1024 /home/hadoop/xubo/tools/cloud-scale-bwamem-0.2.1/target/cloud-scale-bwamem-0.2.0-assembly.jar cs-bwamem -bfn 1 -bPSW 1 -sbatch 10 -bPSWJNI 1  -oChoice 2 -oPath hdfs://219.219.220.149:9000/xubo/data/alignment/cs-bwamem/fastq/g38L100c10000000Nhs20Paired12F2.adam -localRef 1 -jniPath /home/hadoop/xubo/tools/cloud-scale-bwamem-0.2.1/target/jniNative.so -isSWExtBatched 1  1 /home/hadoop/cloud/adam/xubo/data/GRCH38Sub/cs-bwamem/GRCH38BWAindex/GRCH38chr1L3556522.fasta  /xubo/data/alignment/cs-bwamem/fastq/g38L100c10000000Nhs20Paired12.fastq

5.merge:

spark-submit --executor-memory 6g --class cs.ucla.edu.bwaspark.BWAMEMSpark --total-executor-cores 20 --master spark://219.219.220.149:7077  --conf spark.driver.host=219.219.220.149 --conf spark.driver.cores=4 --conf spark.driver.maxResultSize=6g --conf spark.storage.memoryFraction=0.7  --conf spark.akka.threads=2 --conf spark.akka.frameSize=1024 /home/hadoop/xubo/tools/cloud-scale-bwamem-0.2.1/target/cloud-scale-bwamem-0.2.0-assembly.jar merge hdfs://219.219.220.149:9000 /xubo/data/alignment/cs-bwamem/fastq/g38L100c10000000Nhs20Paired12F2.adam /xubo/data/alignment/cs-bwamem/fastq/g38L100c10000000Nhs20Paired12F2.merge.adam

6.统计:

    package org.bdgenomics.avocado.cli    import org.apache.spark.sql.SQLContext    import org.apache.spark.{SparkConf, SparkContext}    /**      * Created by xubo on 2016/5/27.      * 从hdfs下载经过avocado匹配好的数据      * run:success      */    object parquetRead2csbwamemDup {      def main(args: Array[String]) {        val conf = new SparkConf().setMaster("local[4]").setAppName(this.getClass().getSimpleName().filter(!_.equals('$')))        val sc = new SparkContext(conf)        val sqlContext = new SQLContext(sc)        println("start:")        //    val file = "hdfs://219.219.220.149:9000/xubo/16.adam/0"        //    val file = "hdfs://219.219.220.149:9000/xubo/data/alignment/cs-bwamem/fastq/g38L100c10000000Nhs20.merge.adam/"        val file = "hdfs://219.219.220.149:9000/xubo/data/alignment/cs-bwamem/fastq/g38L100c10000000Nhs20Paired12F2.merge.adam/"        val df3 = sqlContext.read.option("mergeSchema", "true").parquet(file)        //    df3.printSchema()        df3.show()        println(df3.count())        var j = 1        //    for (i <- df3) {        //      println(j + ":" + i)        //      j += 1        //    }        //    println("distinct:" + df3.distinct.count())        sc.stop        println("end")      }    }

结果:

    scala>     println(df3.count())    18512773    scala> println("distinct:" + df3.distinct.count())    distinct:18512773   

附录:
部分记录:

    analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.56094168004282, 9.939863106970265)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.56094168004282 9.939863106970265    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.3929171883384, 9.895888368589208)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.3929171883384 9.895888368589208    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.5008221332571, 9.893101752855983)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.5008221332571 9.893101752855983    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.50341742708892, 9.938821490084942)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.50341742708892 9.938821490084942    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.43662172257996, 9.956950171714343)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.43662172257996 9.956950171714343    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.5294684266857, 9.903683785238687)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.5294684266857 9.903683785238687    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.40190347187567, 9.937420104198324)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.40190347187567 9.937420104198324    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.45073240443014, 10.004935473704805)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.45073240443014 10.004935473704805    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.48925135028793, 9.872713569624056)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.48925135028793 9.872713569624056    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.40273561658512, 9.90951049687331)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.40273561658512 9.90951049687331    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.46782169411932, 9.994092930915187)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.46782169411932 9.994092930915187    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.4832529043789, 9.916498387414416)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.4832529043789 9.916498387414416    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.51942615912995, 9.980650734352077)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.51942615912995 9.980650734352077    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.42283366088975, 10.025277342223173)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.42283366088975 10.025277342223173    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.56863795443184, 9.884954572476323)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.56863795443184 9.884954572476323    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.54418222714128, 9.937606729429495)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.54418222714128 9.937606729429495    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.51789788933257, 9.908230182966792)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.51789788933257 9.908230182966792    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.49175824175825, 9.953633581247528)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.49175824175825 9.953633581247528    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.57901688501767, 9.95075215935561)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.57901688501767 9.95075215935561    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.47238496713348, 9.984373782742304)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.47238496713348 9.984373782742304    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.46635524016688, 9.968761617488683)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.46635524016688 9.968761617488683    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.38825547732228, 9.92027510738216)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.38825547732228 9.92027510738216    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.4851967207246, 9.937352774317741)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.4851967207246 9.937352774317741    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.55340706386016, 9.817553555623133)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.55340706386016 9.817553555623133    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.44619450694668, 9.920089825716484)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.44619450694668 9.920089825716484    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.5452329749104, 9.926594228354224)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.5452329749104 9.926594228354224    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.45703727621714, 9.948262102053539)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.45703727621714 9.948262102053539    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.4996774887121, 9.889445679734042)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.4996774887121 9.889445679734042    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.44845544695727, 10.04637179033811)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.44845544695727 10.04637179033811    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.54115079081086, 9.861927310088358)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.54115079081086 9.861927310088358    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.50392688847637, 9.923422645866905)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.50392688847637 9.923422645866905    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.47992413398225, 9.938359869833343)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.47992413398225 9.938359869833343    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.58786034019695, 9.881771439959131)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.58786034019695 9.881771439959131    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.53944973771544, 9.928751173123029)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.53944973771544 9.928751173123029    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.51932833154697, 9.921047819212657)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.51932833154697 9.921047819212657    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.5051575828961, 9.955724349633446)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.5051575828961 9.955724349633446    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.45987665323875, 9.934862525129335)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.45987665323875 9.934862525129335    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.41697601370646, 9.924344174968864)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.41697601370646 9.924344174968864    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.4504008016032, 10.037075657682921)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.4504008016032 10.037075657682921    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.53644939965696, 9.99170757810556)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.53644939965696 9.99170757810556    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.48251923421006, 9.977411795227688)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.48251923421006 9.977411795227688    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.50750643408637, 9.996386292381906)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.50750643408637 9.996386292381906    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.43163108941235, 9.93712833366825)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.43163108941235 9.93712833366825    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.4462753788962, 9.969547663168125)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.4462753788962 9.969547663168125    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.564805511698, 9.981653834837397)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.564805511698 9.981653834837397    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.36601983305985, 9.935551164661065)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.36601983305985 9.935551164661065    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.5214522629195, 9.948307577425197)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.5214522629195 9.948307577425197    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.4983700519434, 9.945836975580079)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.4983700519434 9.945836975580079    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.55142989753293, 9.949033183252514)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.55142989753293 9.949033183252514    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.57046907934694, 9.951195165671601)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.57046907934694 9.951195165671601    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.5627628483855, 9.940990579395123)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.5627628483855 9.940990579395123    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.57557661362418, 9.88907098626934)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.57557661362418 9.88907098626934    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.43170531280145, 9.97153529406552)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.43170531280145 9.97153529406552    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.50119476443524, 9.993324406109274)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.50119476443524 9.993324406109274    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.53636818766986, 9.949249012517377)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.53636818766986 9.949249012517377    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.56565548290277, 9.982952754471368)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.56565548290277 9.982952754471368    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.44116064703562, 9.922618947362455)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.44116064703562 9.922618947362455    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.48944921316166, 9.932084984684073)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.48944921316166 9.932084984684073    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.3957828750534, 9.897098539777856)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.3957828750534 9.897098539777856    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.46895465769603, 9.819260659759172)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.46895465769603 9.819260659759172    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.5120634807163, 9.905862404052492)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.5120634807163 9.905862404052492    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.50274353310056, 9.979749125168556)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.50274353310056 9.979749125168556    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.49695820211852, 9.940759507891329)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.49695820211852 9.940759507891329    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.42262032467997, 9.919691202862072)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.42262032467997 9.919691202862072    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.49617638650656, 9.973742669699334)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.49617638650656 9.973742669699334    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.57427937915742, 9.952728233473406)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.57427937915742 9.952728233473406    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 198, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.48755438271164, 9.944608296251676)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.48755438271164 9.944608296251676    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.52546792398914, 10.000808728139058)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.52546792398914 10.000808728139058    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (192, 199, 205)    low and high boundaries for computing mean and std.dev: (166, 231)    mean and std.dev: (198.49446902654867, 9.999977060170599)    low and high boundaries for proper pairs: (153, 244)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 153 244 0 198.49446902654867 9.999977060170599    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Load FASTQ files                                                                    @Worker1    skip orientation FF as there are not enough pairs                                   analyzing insert size distribution for orientation    (25, 50, 75) percentile: (191, 198, 205)    low and high boundaries for computing mean and std.dev: (163, 233)    mean and std.dev: (198.28641975308642, 10.159216233717126)    low and high boundaries for proper pairs: (149, 247)    skip orientation RF as there are not enough pairs    skip orientation RR as there are not enough pairs    @MemPeStat    pes(0): 0 0 1 0.0 0.0    pes(1): 149 247 0 198.28641975308642 10.159216233717126    pes(2): 0 0 1 0.0 0.0    pes(3): 0 0 1 0.0 0.0    Summary:    Worker1 Time: 815792    Calculate Metrics Time: 2093    Worker2 Time: 688463    16/06/03 18:07:19 ERROR LiveListenerBus: SparkListenerBus has already stopped! Dropping event SparkListenerExecutorMetricsUpdate(4,WrappedArray())    16/06/03 18:07:19 WARN QueuedThreadPool: 1 threads could not be stopped    CS-BWAMEM Finished!!!    Jun 3, 2016 5:41:47 PM INFO: parquet.hadoop.ParquetInputFormat: Total input paths to process : 21    Jun 3, 2016 5:41:57 PM INFO: parquet.hadoop.ParquetInputFormat: Total input paths to process : 21    hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub/cs-bwamem$ 

参考

【1】https://github.com/xubo245/AdamLearning【2】https://github.com/bigdatagenomics/adam/ 【3】https://github.com/xubo245/SparkLearning【4】http://spark.apache.org

研究成果:

【1】 [BIBM] Bo Xu, Changlong Li, Hang Zhuang, Jiali Wang, Qingfeng Wang, Chao Wang, and Xuehai Zhou, "Distributed Gene Clinical Decision Support System Based on Cloud Computing", in IEEE International Conference on Bioinformatics and Biomedicine. (BIBM 2017, CCF B)【2】 [IEEE CLOUD] Bo Xu, Changlong Li, Hang Zhuang, Jiali Wang, Qingfeng Wang, Xuehai Zhou. Efficient Distributed Smith-Waterman Algorithm Based on Apache Spark (CLOUD 2017, CCF-C).【3】 [CCGrid] Bo Xu, Changlong Li, Hang Zhuang, Jiali Wang, Qingfeng Wang, Jinhong Zhou, Xuehai Zhou. DSA: Scalable Distributed Sequence Alignment System Using SIMD Instructions. (CCGrid 2017, CCF-C).【4】more: https://github.com/xubo245/Publications

Help

If you have any questions or suggestions, please write it in the issue of this project or send an e-mail to me: xubo245@mail.ustc.edu.cnWechat: xu601450868QQ: 601450868
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